engleski

Enzymes involved in osmolyte transport processes in the Adriatic lobster Homarus gammarus

Trasport related enzymes Na, K- ATPase and carbonic anhydrase (CA) were studied in the branchiostegite and antennal gland of the commercially important Adriatic lobster Homarus gammarus acclimated to seawater (SW, 38ppt) and dilute seawater (DSW, 20ppt).In homogenates of branchiostegite from DSW-acclimated lobsters, specific activity of Na, K- ATPase was about 2-fold higher of the native (3.2 mmol Pi/h/mg protein) and saponin treatede homogenates (6.6 mmolPi/h/mg protein) than those of the SW-acclimate lobsters. In DSW acclimated lobsters , CA activity of branchiostegite homogenates was 5.6 -fold of activities reported in SW (4.42 U per mg proteins). In DSW specific activity of the Na, K-ATPase in antennal gland native homogenates was 2-fold increased of the SW lobsters (3.2 mmol Pi/h/mg protein). However, the CA in antennal gland was not activated in DSW keeping the same values as in SW. The result suggest different strategies of the enzyme fitting in the studied osmoregulatory tissues.

lobster; Homarus gammarus; branchiostegite; antennal gland; Na; K-ATPase Carbonic anhydrase

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