engleski

p53 and apoptosis in alloxan-treated rats

Althrough alloxan has been widely used as an experimental diabetogen, alloxan induced apoptosis of pancreatic B-cells was not described until recently. The aim of this study was to investigate weather p53, tumor suppressor protein, and "the guardian" of genome is involved in apoptosis induction after alloxan challenge. A single sc administration of 150 mg/kg of alloxan monohydrate to female Wistar rats resulted in severe hyperglycemia together with clinical features of diabetes. Histological analysis revealed significant arte of apoptosis in HE-stained, paraffin embedded pancreatic section 48 h after alloxan treatment, in contrast to no apoptosis in control rats. Immunohistochemical staining performed by p53 (Ab-7) kits (Onkogene, Calbiochem), showed significant p53 expression as early as 15 min after alloxan administration, lasting 24 h, no staining in controls. the disapperance of p53 might have been involved in triggering of cell dying by apoptosis in alloxan treated rats.

apoptosis; alloxan; p53

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