engleski

Accumulation and removal of cyclobutane pyrimidine dimers in Isochrysis galbana cells exposed to artificial UV and solar irradiation

UV-absorbing compounds production is responsive to illumination regime that is consistent with the protection role of UV-absorbing compounds. Exposure to artificial UVB showed a clear 24 hr repair pattern in I. galbana DNA. No significant influence on CPDs removal was detected in samples recovering under constant illumination in the presence of DNA repair inhibitors (caffeine or nalidixic acid). When cells grown under 16h light : 8hr dark regime were recovering in the dark (no photorepair) and in the presence of nalidixic acid (no nucleotide excision repair) the initial amount of CPDs in DNA remained after 24 hours. I. galbana cells posses DNA repair mechanisms that are efficient in CPDs removal during 3 hrs of sunlight exposure.

CPDs; marine phytoplankton; sunlight; UV-adsorbing compounds; UV light

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