engleski

Effect of indole-3-butyric acid on phytoplasmas in infected Catharanthus roseus shoots grown in vitro

Phytoplasmas are non-cultivable bacteria usually maintained in Catharanthus roseus shoots grown in vitro on MS medium with benzylaminopurine (BA). The aim of our research was to examine the influence of indole-3-butyric acid (IBA) on C. roseus shoots infected with three different phytoplasma strains. Supplement of IBA in the medium supported plant growth, photosynthesis and remission of symptoms in all phytoplasma-infected shoots, but had no effect on the presence of EY-C and SA-I phytoplasma strains in tested tissue. However, HYDB phytoplasma was undetectable in approximately half of the tested shoots grown on the medium with IBA. After one year of IBA treatment, HYDB-infected periwinkle shoots were retransferred to the medium supplemented with BA. Some of the shoots showing remission of symptoms during the IBA treatment permanently escaped the infection and remained negative when tested for phytoplasma presence. This is the first report on differential influence of plant growth regulators on phytoplasma-infected C. roseus shoots.

antiphytoplasmal activity; HYDB phytoplasma strain; indole-3-butyric acid; periwinkle; photosystem II; tissue culture

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