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Satellite DNAs and insect models – Tribolium

The genome of the red flour beetle Tribolium castaneum was recently sequenced and draft assembly that represent about 70 % of the genome was produced. In silico analysis of the abundance and distribution of repetitive DNA revealed that approximately 30% of the assembled genome is composed of repetitive DNA (Wang et al, Genome Biology 2008, 9:R61). In assembled genome satellite DNAs build only 2, 5%. This results is in contrast with experimental data which revealed that highly abundant (peri)centromeric 360 bp satellite makes up17% of the genome. It has been proposed that “ unknown” part of genome represent regions of highly repetitive DNA that could not be assembled. Understanding the organization and features of this kind of sequences can help efforts to gain a complete picture of genome structure. Therefore, I will present our preliminary in silico and experimental analysis of newly identified satellite DNAs from reptigs that failed to assemble into scaffolds as well as a comprehensive analysis of 360 bp satellite DNA described previously.

satellite DNA; genome; Tribolium

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