engleski

Transcription of pericentromeric heterochromatin in beetles – satellite DNAs as active regulatory elements

Tenebrionid beetles from the genus Palorus (Coleoptera) have a significant amount of heterochromatin in pericentromeric regions of all chromosomes. Major DNA component of pericentromeric heterochromatin is a highly abundant satellite DNA. Analysis of transcription of a major satellite DNA PSUB from species Palorus subdepressus reveals constitutive level of expression similar in all three developmental stages: larvae, pupae and adults, corresponding to 0.01% of total RNA. Transcription proceeds from both DNA strands in equal amount resulting in long heterogeneous size transcripts ranging in size from 500 bp to more than 5 kb. Although equal transcription from both DNA strands could potentially activate the RNA interference (RNAi) pathway no processing of long PSUB transcripts into small interfering RNAs (siRNA) was detected. Transcripts preferentially remain in the nucleus and 90% of them are not polyadenylated. Transcription initiation sites are located within PSUB satellite monomer as well as motifs characteristic for RNA polymerases II and III. Putative RNA polymerase II promoter shares a 65% sequence similarity to Pol II promoter mapped previously in PRAT satellite DNA, major satellite of related species P. ratzeburgii. Results give strong indication that Palorus satellite DNAs are transcribed as autonomous transcription units from own promoters that reside within satellite sequences. Long satellite DNA transcripts remain mostly in the nucleus and are proposed to play a structural role in the organization of pericentromeric heterochromatin. Using RNAi assay underexpression of satellite DNA is induced and its influence on distribution of heterochromatin proteins HP1 and histone H3K9me2, as well as on Aurora B is studied by immunofluorescence.

satellite DNA ; transcription ; centromere

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