engleski

Carbonic anhydrase activity on total esterase activity level in gills of mussel Mytilus galloprovincialis: a field study

In this study the esterase activity of carbonic anhydrase (CA) on total esterase activity level was investigated in mussels sampled from 19 locations along the Croatian coast of Adriatic Sea. The gills were the target tissue, because the respiratory, ionic transport and pH regulatory enzyme function of CA and its potential usage as biomarkers of environmental pollution was the main topic. So far no report has dealt with the esterase activity of CA on total esterase activity level in mussel. Previously it was suggested possible application of CA activity inhibition as an in vitro bioassay for detection of heavy metals in pollution monitoring using Mediterranean mussel Mytilus galloprovincialis Lamarck, 1819. Total esterase activity was measured in cytosolic fraction of gills homogenates by colorimetric end point reaction using p-nitrophenyl acetate as enzyme substrate, and CA activity was estimated by the same enzymatic reaction using acetazolamide as a specific CA inhibitor (CAII etc). The results of total esterase activities in winter (March ; average value 0.137±0.057) were lower then determined for summer season (August ; 0.153±0.036) at almost all investigated locations. CA activities determined in gills of mussel sampled in winter ranged from 1.75% to 24.65% of total esterase activity. CA activities in summer samples were practically not detectable. This is preliminary research, and it is difficult to assume direct relationship of pollution at investigated sites with determined CA activities, especially in summer period. Despite in vitro studies showed clear inhibition of CA activity with heavy metals, for application of CA as biomarkers of pollution further research of environmental and physiological factors effects and different CA isozymes activity using different inhibitors, protein characterization and sequencing are needed.

Total esterase activity; carbonic anhydrase; Mytilus galloprovincialis; Adriatic Sea

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