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Rb1 mRNA and protein expression in sporadic colon cancer

Accumulation of mutations in normal colon epithelium leads to malignant transformation of the tissue resulting in colon cancer. This process of colorectal carcinogenesis involves activation of oncogenes and inactivation of tumor suppressor genes. One of the gatekeeper genes that are considered to be involved in the development of colon cancer is Rb1. The aim of our study was to evaluate changes in Rb1 at different levels in the cell in order to elucidate its role in colon cancer. We examined the levels of Rb1 mRNA and protein expression in sporadic colon cancer. Rb1 mRNA expression was analyzed in 50 pairs of normal and corresponding tumour tissue samples using real-time PCR method. Rb1 protein expression was analyzed on 50 tumour paraffin sections using immunohistochemistry. Expression of Rb1 mRNA was higher in moderate and poorly differentiated tumours and tumours classified as Dukes' C. Rb1 protein was immunohistochemically positive in 89% of examined tumour samples. The expression of pRb was found higher in moderate and poorly differentiated tumours and it positively correlated with Dukes stage. Our results support the thesis that malignant transformation in colon tissue is a consequence of more than one genetic alteration and suggest that Rb1 plays a role in this multistep process.

Rb1; expression; sporadic colon cancer

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