engleski

The optimization of the lipid hydroperoxide assay

Radical-mediated oxidation products of lipids have been studied for many years though the use of the products of these reactions as specific markers of oxidative damage in vivo has been developed only in the past decade. The greatest needs in this field of research has been to develop relible methods for the quantitative measurement of oxidative products. One of these products are detrimental lipid hydroperoxides (LOOH) which are well known to initiate further autoxidations during reactions of their production and decomposition. Quantitative analysis of LOOH have been in a demand as a consequence in an effort to provide the method for determination of LOOH. The spectrophotometric method of analysis of LOOH was based on the oxidation of ferrous to ferric ion and subsequent complexation of ferric ion by thiocyanate. The aim of this work was a reestimation of the oxidation of ferrous ion by hydroperoxide of oleic and linoleic acid to optimize the assay conditions with special attention given to the rate of oxidation of ferrous ion with alkyl hydroperoxide of different structure, thus improving acceleration of the oxidation and the sensitivity of the method.

Analytic method; Lipid hydroperoxyde; Ferritiocynate method

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