engleski

Paraoxonase and arylesterase activities in the serum of two hyperlipoproteinaemic patients after repeated extracorporal lipid precipitation

The effect of heparin-induced extracorporal lipid precipitation (HELP) on the activities of total paraoxonase (EC 3.1.8.1 ) and of EDTA-sensitive (v-sen) and EDTA-insensitive (v-ins) arylesterase (EC 3.1.1.2) was studied in serum of a patient with hyperlipoproteinaemia (A) and in a patient with non-insulin dependent diabetes mellitus and hyperlipoproteinaemia (B). The enzyme activities were measured spectrophotometrically (Tris/HCI buffer, pH=7.4, 37°C) with paraoxon and phenylacetate as substrates of paraoxonase and arylesterase resp. Both patients underwent HELP applications once a week over a period of 7 weeks. Over that period no overall change was observed either in enzyme activities or in the lipid or protein content of the serum samples. However, each HELP session caused an immediate decrease of v-ins (on average 56% in A and 42% in B), while v-sen arylesterase remained almost unaltered. Paraoxonase remained unchanged in A, but decreased in B (about 60% ). Of the atherogenic parameters, the most pronounced decrease was found in VLDL-cholesterol and in triglycerides (on average 45% in A and 32% in B), while the anti-atherogenic HDL-cholesterol decreased less than 10%. Possible implications of the effect of HELP on the studied enzyme activities remain to be explained.

serum paraoxonase; serum arylesterase; hyperlipoproteinaemia

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