engleski

Flavonoid Content Assay: Prevalidation and Application on Plantago L. Species

This work is aimed to prevalidate and apply UV/Vis spectrophotometric procedure for flavonoid determination in herbal material using aluminium chloride as a reagent. Fast and simple, full prevalidation for quality contol and standardization od analytical procedure, based on mathematical/statistical testing coupled with system of diagnosis was used to evaluate and demonstrate the reliability of method for flavonoid determination with aluminium chloride (F-Al procedure). Favourable prevalidation characteristics verified this procedure as a valuable tool in flavonoid analysis, so it was successfully applied for determination of flavonoids in leaves, stems and flowers of Plantago L. species growing in Croatia. The results showed the various flavonoid contents between different plant parts (leaves: up to 0.13% ; stems: up to 0.07% and flowers: up to 0.07%).The results of flavonoid determination were statistically evaluated by using Principal Component Analysis (PCA) and Student's t-test as a contribution to investigation of different taxa of genus Plantago L.

prevalidation strategy; flavonoids; aluminium chloride; Plantago L.

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