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Development and prevalidation of a method for phenol determination by solid-phase spectrophotometry (CROSBI ID 138840)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Vuković, Jadranka ; Matsuoka, Shiro ; Yoshimura, Kazuhisa ; Grdinić, Vladimir ; Jurišić Grubešić, Renata Development and prevalidation of a method for phenol determination by solid-phase spectrophotometry // Mikrochimica acta (1966), 159 (2007), 3-4; 277-285. doi: 10.1007/s00604-007-0748-5

Podaci o odgovornosti

Vuković, Jadranka ; Matsuoka, Shiro ; Yoshimura, Kazuhisa ; Grdinić, Vladimir ; Jurišić Grubešić, Renata

engleski

Development and prevalidation of a method for phenol determination by solid-phase spectrophotometry

A simple, sensitive and rapid solid-phase spectrophotometric procedure was developed for the determination of traces of phenol with 4-aminoantipyrine as a reagent (AAP-SPS), and the optimal experimental conditions were established. This method was performed by sorption and direct absorbance measurements of the product phenol-AAP sorbed on the anion-exchanger Dowex 1-X4 (0.2 g) at 495 nm (absorption maximum) and 700 nm (non-absorption wavelength). The sensitivity offered by the AAP-SPS procedure was higher by a factor of 40 compared with the respective conventional spectrophotometric method. Metrological characteristics were established using a prevalidation strategy. The AAP-SPS procedure is characterized by a linear calibration function in the working range of 0.05-0.50 mu mol, low standard deviation of procedure (+/- 0.012), low limit of determination (0.021 mu mol), and favorable random (+/- 0.85 to +/- 11.27%) and systematic deviations (-4.55 to +/- 11.50%). Moreover, the accuracy of the system investigated by the recovery test is acceptable (99-102%). Favorable working and performance characteristics make the new SPS method ideal for phenol monitoring in pharmaceutical preparations as well as other matrices.

phenol determination; prevalidation strategy; solid-phase spectrophotometry; 4-aminoantipyrine; pharmaceutical application

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Podaci o izdanju

159 (3-4)

2007.

277-285

objavljeno

0026-3672

10.1007/s00604-007-0748-5

Povezanost rada

Kemija, Farmacija

Poveznice
Indeksiranost