engleski

Suvremena laboratorijska dijagnostika alergije

The requests for laboratory diagnosis of allergic diseases refer to a battery of tests that should provide the following: 1) determine the type of allergic reaction by detecting humoral (total IgE) and cellular (eosinophilic and basophilic granulocyte count, eosinophil cationic protein) mediators of allergic reaction ; 2) identify triggers of allergic reaction (specific IgE to particular causative allergens, tests for basophilic granulocyte ex vivo activation, leukotrienes, CD63, histamine) ; 3) assess the clinical course of allergic reaction (tryptase for immediate reaction, eosinophil cationic protein for delayed reaction) ; 4) enable specific immunotherapy monitoring (total and specific IgE, specific IgG4 and IgG1, IgG4/IgG1 ratio, proallergic and proinflammatory cytokines) ; and 5) estimate diagnostic efficiency of determination of particular humoral or cellular mediators of allergic reaction (i.e., sensitivity, specificity, positive and negative predictive value). Althouh laboratory investigations are a useful tool in the diagnosis and management of allergic diseases many problems remain unsolved. Those problems can be grouped into three categories: preanalytical (specimen collection), analytical and post-analytical (interpretation, follow-up, retesting) phases of laboratory testing.

IgE; bazofilni granulociti; leukotrijeni; imunoterapija

nije evidentirano