engleski

In vitro regeneration of Croatian endemic species Iris adriatica Trinajstić ex Mitić

Plant regeneration via somatic embryogenesis and organogenesis was achieved in leaf base and ovary culture of the Croatian endemic Iris adriatica Trinajstić ex Mitić. Callus induction from leaf base explants occurred in the dark on three media with MS mineral solution containing 4.52 μM dichlorophenoxyacetic acid (2, 4-D), 4.83 μM naphthaleneacetic acid (NAA), 0.46 μM kinetin (Kin), 5% sucrose and 200 mg L-1 casein hydrolysate. The media differed only in vitamin and/or proline content. Calli from ovary culture were achieved on MS medium containing 45.25 μM 2, 4-D. The mean percentage of callus induction from leaf base explants was 18.9%, with no significant differences between media, and 27.3% from ovary sections. All embryogenic calli were formed on MS media containing 0.45 μM 2, 4-D, 4.44 μM benzyladenine (BA) and 0.49 μM indole-3-butyric acid (IBA) under low light intensity (25 μE m-2s-1). Transfer of embryogenic calli to hormone-free medium enabled the development of mature somatic embryos on the surface of 6.0% of induced calli produced from leaf base explants and 4.0% of those from ovary sections. Genotype had the main effect on plant regeneration efficiency in Iris adriatica.

Iris adriatica; leaf base culture; ovary culture; somatic embryogenesis

nije evidentirano