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RFLP analysis of cpDNA in the genus Hypericum (CROSBI ID 155729)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Hazler Pilepić, Kroata ; Morović, Miranda ; Orač, Filip ; Šantor, Marija ; Vejnović, Vanja RFLP analysis of cpDNA in the genus Hypericum // Biologia (Bratislava), 65 (2010), 5; 805-812. doi: 10.2478/s11756-010-0100-0

Podaci o odgovornosti

Hazler Pilepić, Kroata ; Morović, Miranda ; Orač, Filip ; Šantor, Marija ; Vejnović, Vanja

engleski

RFLP analysis of cpDNA in the genus Hypericum

The chloroplast DNA of 43 species including 16 sections from the genus Hypericum was studied by PCR-RFLP analysis. The PCR-amplified products of four cpDNA regions, trnC-trnD, psbC-trnS, trnL-trnF and rbcL were digested with four restriction endonucleases. A high level of interspecific variation was detected while intraspecific diversity was not observed. The resulting parsimony analysis indicated the monophyletic assemblage of the sections Androsaemum, Olympia, Drosocarpium and Trigynobrathys. Monophyly of Hypericum is weakly supported, but close relationships of H. perforatum and H. maculatum are indicated. The members of Ascyreia are weakly resolved, but clustering of H. kouytchense and H. oblongifolium is well supported, however, H. reptans is nested with Olympia. CpDNA profiles and the positions on the parsimony tree indicate that the chloroplast donor among the putative parents of the hybrid species H. × inodorum is H. androsaemum.

Hypericum ; cpDNA ; PCR-RFLP ; phylogeny

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Podaci o izdanju

65 (5)

2010.

805-812

objavljeno

0006-3088

10.2478/s11756-010-0100-0

Povezanost rada

Farmacija, Biologija

Poveznice
Indeksiranost