engleski

Quantitative analysis of the ear-leaf nitrogen contents in maize inbred lines and their F1 hybrids

Nutrient uptake by plants is under considerable genetic influence (Saric 1981 ; Kovacevic 1984). Six maize inbred lines (Os657, ND301, ND246, Os138, Os77 and Os656) and their 30 hybrids (complete diallel crossing) were grown on Osijek eutric cambisol (three replications, exp. plot 7m2). Based on their the ear-leaf nitrogen status, they were estimated and analyzed GCA and SCA genetic parameters (Table 2). Also, they were made Vr and Wr regression analysis, as well as components of variance and heritability (Singh et al. 1993). Considerable genetic influence on plant N status in maize was found by our testing (Tab. 1 and 2). Table 1. Genetic influences of nitrogen status in six maize inbred lines and their F1 hybrids Father MotherOs657ND301ND246Os138Os77Os656Mean* The ear-leaf nitrogen concentrations (% on dry matter basis) at beginning of the silking stage (middle of July) Os657 ND301 ND246 Os138 Os77 Os6564.29 4.02 3.76 3.78 4.01 3.803.81 4.10 3.65 3.70 3.95 3.563.91 3.79 3.88 3.72 4.00 3.713.71 3.64 3.68 3.72 3.80 3.533.88 3.77 3.77 4.00 4.40 3.613.77 3.61 3.60 3.72 3.77 3.883.90 (3.82) 3.82 (3.77) 3.72 (3.69) 3.77 (3.78) 3.99 (3.90) 3.68 (3.64) Mean* 3.94 (3.87)3.79 (3.73)3.83 (3.83)3.68 (3.67)3.90 (3.81)3.72 (3.69) * in the bracket: means of hybrids (in total 5) only Significant higher GCA values were found by Os 77 and Os 657, compared to the other tested inbred lines (Table 2). Significant specific effects were estimated for Os 77 x Os 138, Os 656 x Os 77 and Os 656 x Os 138 combinations. Significant differences in CA for reciprocal crosses showed reciprocal effects in inheritance of the trait investigated. Also, Simic (1994) estimated high significant reciprocal effects by quantitative analysis of yield and its components. Analysis of components of genetic variance (according to Mather and Jinks 1971) showed predominance of dominant component of genetic variance (overdominance, H1/D=1.624), which was also confirmed by dominant genes frequency (U=0.514). For Vr Wr regression, there was no significant difference from 1 (b = 0.85 0.2034). Heritability estimation (h2=0.8597) was done followed Singh et al. (1993) based on heredity varying portion in total phenotype variability among tested hybrids. Table 2. GCA (general combining ability) and SCA (specific combining ability) effects for the ear-leaf N status Os657ND301ND246Os138Os77Os656 Os657 ND301 ND246 Os138 Os77 Os6560.105 -0.105 0.075 -0.035 -0.065 -0.0150.001 -0.005 0.070 -0.030 -0.090 0.025-0.050 -0.054 -0.035 -0.020 -0.115 -0.055-0.087 -0.051 0.008 -0.087 0.100 0.095-0.107 -0.081 -0.027 0.040 0.133 0.080-0.024 -0.113 -0.014 0.009 -0.146 -0.111 LSDGCA 0.05 = 0.025 LSDSCA 0.05 = 0.078 LSDGCA 0.01 = 0.033 LSDSCA 0.01 = 0.104 References: Kovacevic V. (1984):The ear-leaf percentage of Ca and Mg in maize inbred lines and their diallel progeny. Theor. Appl. Gen. 68:521-523. Saric M. (1981): Genetic specifity in relation to plant mineral nutrition. J. Plant Nutrition 3, 743-766. Singh M., Ceccarelli S., Hamblin J. (1993): Estimation of heritability from varietal trials. Theor. Appl. Genet. 86:437-441. Simic D. (1994): Griffing, s method of diallel analysis in testing of new maize inbred lines (Master Thesis), Fac. of Agric. Zagreb.

maize ; genotypes ; leaf ; nitrogen

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