Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

A new potentiometric sensor for the determination of α-amylase activity (CROSBI ID 169481)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Sakač, Nikola ; Sak-Bosnar, Milan ; Horvat, Marija ; Madunić-Čačić, Dubravka ; Szechenyi, Aleksandar ; Kovacs, Barna A new potentiometric sensor for the determination of α-amylase activity // Talanta, 83 (2011), 5; 1606-1612. doi: 10.1016/j.talanta.2010.11.053

Podaci o odgovornosti

Sakač, Nikola ; Sak-Bosnar, Milan ; Horvat, Marija ; Madunić-Čačić, Dubravka ; Szechenyi, Aleksandar ; Kovacs, Barna

engleski

A new potentiometric sensor for the determination of α-amylase activity

A platinum redox sensor for the direct potentiometric determination of α-amylase concentration has been described. The sensor measured the amount of triiodide released from a starch-triiodide complex, which was correlated with the α-amylase activity after biocatalytic starch degradation. The composition and stability of the potassium triiodide solution was optimized. The starch-triiodide complex was characterized potentiometrically at variable starch and triiodide concentrations. The response mechanism of the platinum redox sensor towards α-amylase was proposed and the appropriate theoretical model was elaborated. The results obtained using the redox sensor exhibited satisfactory accuracy and precision and good agreement with a standard spectrophotometric method and high-sensitive fully automated descret analyser method. The sensor was tested on pure α-amylase (EC 3.2.1.1, Fluka, Switzerland), industrial granulated α-amylase Duramyl 120 T and an industrial cogranulate of protease and α-amylase Everlase/Duramyl 8.0 T/60 T. The detection limit was found to be 1.944 mU for α-amylase in the range of 0-0.54 U (0-15 μg), 0.030 mKNU for Duramyl 120 T in the range of 0-9.6 mKNU (0-80 μg) and 0.032 mKNU for Everlase/Duramyl 8.0 T/60 T in the range of 0-9.24 mKNU (0-140 μg).

α-amylase; potentiometric sensor; starch; triiodide

The authors gratefully acknowledge the financial support of the Croatian Ministry of Science, Education and Sports given to project No. 291-0580000-0169.

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

83 (5)

2011.

1606-1612

objavljeno

0039-9140

10.1016/j.talanta.2010.11.053

Povezanost rada

Kemija, Kemijsko inženjerstvo, Biotehnologija

Poveznice
Indeksiranost