engleski

The relationship between platelet parameters and platelet serotonin level in healthy population

Background. The most frequently described platelet parameters are mean platelet volume (MPV), plateletcrit (Pct) and platelet distribution width (PDW). The issue of possible association of platelet anatomic parameters with serotonin level in the lysates of peripheral blood platelets has not been investigated. The aim of this study is to investigate this association in physiological conditions. Methods. We determined platelet parameters on Beckman Coulter LH 750 haematology analyzer in healthy volunteers (n=143) of both sexes. Analysis has been done for all samples two hours after blood smapling.In addition, we prepared platelet rich plasma(PRP) and optimizated conditions for determination of serotonin content in the lysates.The samples of PRP lysates were frozen at -20° C ; the measurement were performed within two weeks using ELISA.The content of circulatory serotonin in platelets is refered to 109 platelets.The correlations with serotonin concentration and platelet parameters were analyzed by ANOVA. RESULTS :The criterias in our statistical model were: significance level (F=7.5 ; p<=0.001), significant negative correlationbetween platelet serotonin level and MPV(p=0, 03), significant positive correlationbetweenplatelet serotonin level and Pct (p=0, 001) and no correlation betweenplatelet serotonin and PDW (P=0, 346). Conclusions. Our results suggests that a serotonin level is higer in smaller platelets as a result of their aging, and it is not associated with cell size distribution. Under normal coditions , there is an inverse relationship between platelet size and number, so it is possible that serotonin level is higher if the total platelet mass is higher.

platelets; serotonin; platalets parametars

nije evidentirano