The effect of three irrigation techniques on the reduction of intracanal Enterococcus faecalis ex vivo (CROSBI ID 575477)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Bago, Ivona ; Toljan, Ivana ; Baraba, Anja ; Miletić, Ivana ; Anić, Ivica
engleski
The effect of three irrigation techniques on the reduction of intracanal Enterococcus faecalis ex vivo
Aim To compare intracanal bacterial reduction promoted ex vivo by three irrigation techniques with regard to the standardized volume of NaOCl and standardized irrigation time. Methodology Forty-eight single-rooted teeth with straight canals of approximately the same volume were instrumented with ProTaper istruments and sterilized in plasma. Forty-two root canals were innoculated with an Enterococcus faecalis suspension, and the remaining six canals were filled with sterile broth and served as negative controls. After 48 h of incubation, 36 root canals were randomly distributed into 3 groups of 12 roots each according to the irrigation technique used ; group 1, conventional syringe irrigation using needle ; group 2, automated-dynamic irrigation using RinsEndo ; group 3, passive ultrasonic irrigation (PUI) using Piezon Master 400. The remaining six root canals served as positive controls. Antimicrobial efficacy of the irrigation techniques was analysed in regard of standardized volumes of 3% NaOCl (20 mL) in the first protocol, and in regard of standardized irrigation time (45sec) in the second protocol. Samples taken immediately after irrigation were cultured and the colony-forming units (CFUs) were counted. Results When the volume of the irrigant was standardized, RinsEndo was more efficacious than PUI (p<0.01), however, there were no statistically significant differences between other techniques. When the irrigation time was standardized, there were no significant differences between any irrigation technique (p>0.05). The highest percentage of minimal counts of E. faecalis CFUs was noted in RinsEndo group in both protocols. Conclusions On the basis of this ex vivo investigation, RinsEndo was superior to PUI only when the volume of the irrigant was standardized. However, RinsEndo seemed to be more beneficial because it caused higher bacterial reduction in both protocols when using the least amount of irrigant, and provided longer contact time.
Enterococcus faecalis; irrigation; sodium hypochlorite
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Podaci o prilogu
1186-x.
2011.
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objavljeno
Podaci o matičnoj publikaciji
International endodontic journal
Dummer P.M.H.
0143-2885
Podaci o skupu
15. Biennial Congress of the European Society of Endodontology
poster
14.09.2011-17.09.2011
Rim, Italija