engleski

Physical properties of enamel treated with CPP-ACP and fluorides

Objectives: This study was carried out to determine the effect of NaF, TiF4 and CPP-ACP (Tooth Mousse, Recaldent®, GC) applications on artificial white spot lesions in vitro. In order to assess remineralization potential of those agents, a fluoride-uptake, CSMH, SEM and EDX measures were performed. Methods: 75 samples of intact human premolars were expose to pH-cycling during 3 hours in demineralizing solution (pH=4, 3) and 21 hours in artificial saliva (pH=7, 2) during 8 days to create a white spot lesion. The remaining sound enamel was covered with an acid resistant nail-varnish in color (positive control window). After creation of the artificial lesion one demineralized area of each sample was covered with an nail-varnish in another color (negative control window). Specimens were randomly assigned into 5 experimental groups to obtain 15 samples per group. The groups received the treatments with different agents: 1. NaF solution (1%), 2. TiF4 solution (1%), 3. NaF solution (1%) + CPP-ACP, 4. TiF4 (1%) + CPP-ACP and 5. solely CPP-ACP. Fluoride-uptake was measured in first 4 groups. All samples were bisected in order to obtain two halves througout experimental window and positive and negative control window. Samples were examined with micro-hardness tester by making three indentations on each experimental window. The indentations were 250μm (A), 400 μm (B) and 550 μm (C) of depth. Three selected samples per group were additionally examined with SEM and EDX. Results: Fluoride uptake was the highest in the 2nd and the lowest in the 3rd group. There were no significant differences neither between 5 groups nor between experimental and control groups according to CSMH. EDX shoved significant incorporation of titanium in surface layer of enamel (4, 8 %Wt in 2nd and 7, 4 %Wt in 4th group) (p ≤ 0, 05). Conclusions: Presence of CPP-ACP does not influence physical properties of enamel. 0442

enamel; fluorides; CPP-ACP

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