Diagnosis of Liposcelis entomophila (Insecta: Psocodea : Liposcelididae) based on morphological characteristics and DNA barcodes (CROSBI ID 178138)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Yang, Qianqian ; Kucerová, Zuzana ; Li, Zhihong ; Kalinović, Irma ; Stejskal, Václav ; Opit, George ; Cao, Yang
engleski
Diagnosis of Liposcelis entomophila (Insecta: Psocodea : Liposcelididae) based on morphological characteristics and DNA barcodes
Liposcelis entomophila infests stored grain and is one of the most important psocid species worldwide. Six geographically isolated strains of L. entomophila from Asia, Europe, and United States of America (USA) were compared based on morphological attributes and by molecular methods. Decisive characters of morphological diagnosis were studied using body size measurements and Scanning Electron Microscopy (SEM). Molecular identification of the six strains was performed via identification of DNA sequence similarities and phylogenetic analyses based on a 655-bp fragment from the 50 end of the standard mitochondrial gene cytochrome c oxidase I (COI) barcode region. The results showed that both morphological and molecular approaches were able to accurately identify this species. Kimura-2-Parameter (K2P) divergence between geographically isolated strains was on average 1.75% for the COI sequence. Phylogenetic analyses revealed that sequences of L. entomophila strains’ COI barcodes formed clusters with tight cohesion that were clearly distinct from those of allied species.
storage pests ; electron microscopy ; mtDNA COI ; diagnosis ; Psocoptera
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o izdanju
48
2012.
120-125
objavljeno
0022-474X
1879-1212
10.1016/j.jspr.2011.10.007
Povezanost rada
Poljoprivreda (agronomija)