engleski

Morphometric and molecular analysis of a pink-berried mutant within the population of grape cultivar ‘Plavac mali’

This study reports characteristics of pink-berried mutants found in the clone population of the Croatian red wine cultivar ‘Plavac mali’ on the basis of comparative ampelographic and DNA marker (SSR and Gret 1) analysis. The pink-berried accession, also called ‘Plavac mali sivi’ (Croat. sivi = Eng. grey or French gris), along with the other 58 ‘Plavac mali’ accessions of standard blue-black berry skin color, has been characterized for the first time using OIV descriptors and molecular markers. Using a set of 9 simple sequence repeat (SSR) markers, an identical SSR profile for all the analyzed accessions was revealed, indicating their monozygotic status. The analysis of Gret 1 insertion within the VvMYBA1 locus revealed no DNA polymorphism responsible for the pink-berried phenotype. Surface color of the berry skin was measured with the CIELab technique using a reflectance spectrophotometer at full ripeness. The results of colorimetric variables (L*, a* and b*) suggest a significantly lower accumulation of anthocyanins in the pink-berried accession compared to the standard blue-black berries. The pink-berried accession shares all assessed morphological and genetic traits of ‘Plavac mali’, with the only difference being the color of the berry skin. This suggests that the pink-berried genotype is the result of a spontaneous mutation of a standard ‘Plavac mali’ genotype.

clone; ampelography; anthocyanins; microsatellites; CIELab; Gret1

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