Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi !

Freeze-Drying of Melatonin-Loaded Lecithin/Chitosan Nanoparticles (CROSBI ID 582561)

Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija

Hafner, Anita ; Durrigl, Marjana ; Filipović-Grčić, Jelena Freeze-Drying of Melatonin-Loaded Lecithin/Chitosan Nanoparticles // Scientia Pharmaceutica. 2010. str. 676-676

Podaci o odgovornosti

Hafner, Anita ; Durrigl, Marjana ; Filipović-Grčić, Jelena

engleski

Freeze-Drying of Melatonin-Loaded Lecithin/Chitosan Nanoparticles

Recently, increasing attention has been paid to lecithin/chitosan nanoparticles as colloidal drug delivery system showing the great potential to improve the permeation of encapsulated drug across various biological barriers [1, 2]. However, the overall poor stability of colloidal nanoparticles in an aqueous medium is a significant drawback in their development perspective. Therefore, the aim of this study was to set up a freeze-drying process of melatonin-loaded lecithin/chitosan nanoparticles in order to improve their physico-chemical stability. Suspensions of nanoparticles were freeze-dried in the presence of glucose or trehalose as cryoprotectants. Nanoparticles were characterised in terms of size, polydispersity, zeta-potential and melatonin content before and after the lyophilisation process. Before the lyophilisation process, lecithin/chitosan nanoparticles obtained were characterised by mean diameters ranging between 117.4 ± 3.9 and 328.5 ± 3.1 nm, and were significantly larger than comparable lecithin nanoparticles. The zeta potential was inverted from negative values for lecithin nanoparticles to positive values for lecithin/chitosan nanoparticles (up to 30.2 mV).The size and the surface charge of the nanoparticles increased with the increase in the chitosan content and the negative charge of lecithin used in the preparation. Compared to lecithin nanoparticles, lecithin/chitosan nanoparticles were characterised by significantly higher melatonin loadings (up to 7.1 %, w/w). In the lyophilisation process it was shown that a minimal concentration of 2.5 % (w/v) of trehalose or glucose was needed for a suitable reconstitution of nanoparticles lyophilised at particle concentration of 2 mg/ml. No significant difference in the mean particle size, size distribution or zeta-potential of nanoparticles in suspension before and after freeze-drying and reconstitution were observed. No significant melatonin leakage caused by the stress of freezing and dehydration in the presence of cryoprotectants occurred.

chitosan; lecithin. melatonin; freeze-drying; nanoparticles

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

676-676.

2010.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

Scientia Pharmaceutica

0036-8709

Podaci o skupu

8th Central European Symposium on Pharmaceutical Technology

poster

16.09.2010-18.09.2010

Graz, Austrija

Povezanost rada

Farmacija

Indeksiranost