Galectin-3 expression in response to LPS, immunomodulatory drugs and exogenously added galectin-3 in monocyte-like cells (CROSBI ID 179710)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Dabelić, Sanja ; Novak, Ruđer ; Šupraha Goreta, Sandra ; Dumić, Jerka
engleski
Galectin-3 expression in response to LPS, immunomodulatory drugs and exogenously added galectin-3 in monocyte-like cells
Galectin-3, a structurally unique beta-galactoside binding lectin, through the specific protein-protein and protein-carbohydrate interactions participates in numerous biological processes, such as cell proliferation and apoptosis, adhesion and activation. Its expression and secretion by until now unknown mechanism are modulated by diverse molecules and are dependent on different physiological and pathophysiological conditions. By autocrine and paracrine actions, galectin-3 modulates many immune reactions and affects various immune cells, particularly those of monocyte-macrophage lineage. This is why galectin-3 has recently become an attractive therapeutic target. However, molecular mechanisms of its actions as well as regulatory mechanism of its expression and activation are still largely unknown. In this study, we show that LPS provokes up-regulation of galectin-3 expression on both gene and protein level in monocyte-like THP-1 cells, which can be inhibited by dexamethasone, but not with non-steroidal anti-inflammatory drugs. Although galectin-3 is generally considered to be a pro-inflammatory molecule, here we show that the exogenously added galectin-3 does not affect IL-1β, IL-6, IL-8, IL-10, IL-12p70 and TNF-α production in resting and LPS-activated monocyte-like THP-1 cells on which it binds with the same capacity, nor influences its own gene expression level in those cells.
cytokines; galectin-3; immunomodulatory drugs; LPS; monocyte-like cells
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Podaci o izdanju
48 (8)
2012.
518-527
objavljeno
1071-2690
10.1007/s11626-012-9540-x
Povezanost rada
Temeljne medicinske znanosti, Farmacija, Biologija