engleski

Tricaine methane-sulfonate (MS-222) application in fish anaesthesia

Tricaine methane-sulfonate (MS-222) is one of the most widely used anaesthetics for poikilotherms worldwide. This paper outlines its anaesthetic efficacy and dosage in fish and legislation for its use, fish stress responses to MS-222 anaesthesia, its effect on fish physiology and blood properties, pharmacokinetics, genotoxicity, immune response, potential interference with fish hepatic cytochrome P450 spectra, and its impact on nerve sensitivity. The key questions arising from the available data are analyzed, such as regulatory constraints on its use, the need for the standardization of buffering protocols, and interdependencies of the factors impacting the specific applicative efficacy of MS-222. Current research has provided an abundance of data on MS-222 use in fish, though the applications within these studies are often impractical at the farming level. Therefore, specific emphasis is placed on highlighting application strategies on a practical basis and presenting potential future research on topics that require in-depth analysis (preparation and storage of anaesthetic solutions, preanaesthetic sedation and stress reduction, cortisol response in aquarium fish, toxicity of MS-222 metabolites, and possible immunodepressive properties). Additionally, both from a scientific and practical perspective, we must have a better understanding of its safety margins, induction, immersion and recovery times for many (marine and freshwater, farmed and ornamental) fish species in order to achieve optimal utilization.

MS-222; buffering; dosage; stress; pharmacokinetics

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