engleski

Ex vivo evaluation of genotoxic effects of four dental adhesives on human leukocytes

The use of dental adhesives in everyday dental practice has raised questions about their biological safety. Their biocompatibility is a relevant aspect of the clinical success of these materials. The objective of this study was to evaluate the genotoxicity of dental adhesives ex vivo using a cytogenetic assay. Four materials (AdheSE, G-Bond, Excite, and Adper Single Bond 2) were tested on human peripheral blood leukocytes using a comet assay. Prepared materials were eluted in a saline solution for 1 h, 1 day, and 5 days. The comet assay was used to evaluate primary DNA damage by measuring the tail length and tail intensity. A Kruskall-Wallis non-parametric test was used for the statistical analysis, with the significance level set to p < 0.05. None of the tested dental adhesives revealed a statistically significant increase in the tail length or tail intensity in treated leukocytes, independent of the applied dilution, elution duration, and polymerization form. A slight increase in the tail length and intensity of DNA molecules was observed after 1 and 5 days of the elution period at the lowest dilution (1: 102) for all tested adhesives, only in their non-polymerized form ; however, these results were not statistically significant. Under the conditions used in this study, all adhesives had acceptable biocompatibility in terms of genotoxicity.

biocompatibility ; comet assay ; dental adhesives ; genotoxicity ; leukocytes

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