engleski

Toll-like receptors tlr-2 and tlr-4 gene polymorphisms in patients with cerebral atherosclerosis

Background. Theinnate immunity proteins TLR-2 and TLR-4 are possible connection between immune response and inflammation involved in the pathogenesis of atherosclerosis. Previous studies suggest that tlr-2 and tlr-4 gene polymorphisms could contribute to the differences in the disease development. Materials and methods. A group of patients with cerebral atherosclerosis, evaluated by digital subtraction angiography, with >50% stenosis of cerebral artery (N=47) was compared to the control group (N=27) concerning single nucleotide polymorphisms G/A753 in the tlr-2 gene and A/G299 in the tlr-4 gene, determined by the real-time PCR. Serum proinflammatory cytokines IL-6 and TNF-a, were determined by enzyme imunoassays. Results. Examined polymorphism of tlr-2 gene was present in 3, 7% and 5, 3% subjects of the control and of the cerebral atherosclerosis group, respectively, while polymorphism of tlr-4 in 9, 3% and 8, 5% subjects, respectively. There was no difference in proportions of the polymorphisms between the groups. The concentrations of IL-6 and TNF-a were higher in the cerebral atherosclerosis than in the control group (1, 54 pg/ml, (1, 04-3, 50 pg/ml) vs. 1, 21 pg/ml )0, 45-2, 32 pg/ml), p=0, 048 and (0 pg/ml (0-1, 8 pg/ml) vs. 0 pg/ml, (0-0 pg/ml), p=0, 039, respectively), presented as median (1-3 quartile). Conclusion. Higher concentrations of IL-6 and TNF-a in the cerebral atherosclerosis group are indicators of inherent inflammation. Single nucleotide polymorphisms G/A573 of the tlr-2 and A/G299 of the tlr-4 gene had no influence on the concentrations of circulating IL-6 and TNF-a. The polymorphisms were not significant for the diagnosis of cerebral atherosclerosis with more than 50% stenosis of cerebral arteries.

toll-like receptors; cerebral atherosclerosisBackgroun

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