engleski

Simultaneous RP-HPLC-DAD separation and determination of flavonoids and phenolic acids in Plantago L. species

A rapid reversed-phase high performance liquid chromatographic method was developed and applied for simultaneous separation and determination of flavonoids and phenolic acids in eight Plantago L. taxa (P. altissima L., P. argentea Chaix, P. coronopus L., P. holosteum subsp. depauperata Pilger, P. holosteum Scop. subsp. holosteum, P. holosteum subsp. scopulorum (Degen) Horvatić, P. lagopus L., and P. maritima L.) growing in Croatia. Chromatographic separation was carried out on Zorbax Eclipse XDB-C18 using gradient elution with a water (pH=2.5, adjusted with trifluoroacetic acid) and acetonitrile mixture at 30˚C. The contents of analyzed phenolic compounds (% of the dry weight of the leaves, dw) varied among examined species: rutin (max. 0.024%, P. argentea), hyperoside (max. 0.020%, P. lagopus), quercitrin (max. 0.013%, P. holosteum subsp. holosteum), quercetin (max. 0.028%, P. holosteum subsp. scopulorum), chlorogenic acid (max. 0.115%, P. lagopus), and caffeic acid (max. 0.046%, P. coronopus). Isoquercitrin was determined only in P. argentea (0.020%), while isochlorogenic acid content was below limit of quantification in all investigated species. Multivariate analysis (UPGMA and PCA) showed significant differences in contents of investigated polyphenolic compounds between different Plantago taxa. Accordingly, investigated substances might be employed as good chemotaxonomic markers in the study of complex genus Plantago.

Plantago; flavonoids; phenolic acids; RP-HPLC-DAD system

nije evidentirano