Comparison of spectrophotometric and HPLC methods for determination of carotenoids in foods (CROSBI ID 192127)
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Podaci o odgovornosti
Luterotti, Svjetlana ; Marković, Ksenija ; Franko, Mladen ; Bićanić, Dane ; Madžgalj, Azamela ; Kljak, Kristina
engleski
Comparison of spectrophotometric and HPLC methods for determination of carotenoids in foods
This report is aimed at intra-laboratory and inter-laboratory comparison of the results obtained during spectrophotometric and HPLC analyses of lycopene, beta-carotene and total carotenoids in tomato products and yellow maize flours/grits. Extensive statistical analyses are performed in order to identify the main sources of uncertainties which may occur when using: (i) different techniques/methods/approaches in the same/different laboratories, in various food samples, and (ii) to indicate the facts/conditions under which the biases between results may remain unidentified after applying statistical testing. Our data points to the inertness of t-test to detect significance of differences, particularly at low R values: in general, the higher correlation coefficient, the higher is sensitivity of statistical testing, especially of the paired t-test. Therefore, simple deviation of relationship line slope from unity could be used as additional evaluation parameter. This adds to reliable and objective quality assurance of foods in regard to carotenoids.
carotenoids; tomato products; yellow maize flours/grits; spectrophotometry; HPLC; statistical analysis
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Podaci o izdanju
Povezanost rada
Kemija, Farmacija, Prehrambena tehnologija