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Determination of Flavonoids, Phenolic Acids, and Xanthines in Mate Tea (Ilex paraguariensis St.-Hil.)

Raw material, different formulations of foods and dietary supplements of mate demand control of the content of bioactive substances for which high performance thin layer chromatography (TLC), described here, presents simple and rapid approach for detections as well as quantification. Using TLC densitometry the following bioactive  were identified and quantified: chlorogenic acid  , caffeic acid (1.5 mg/g), rutin  , quercetin  and kaempferol  . The results obtained with TLC densitometry for caffeine  and theobromine  show no statistical difference to the content of total xanthines (7.6 mg/g) obtained by UV-Vis spectrophotometry. Thus, this TLC stays a technique of choice for simple and rapid analysis of great number of samples as well primary screening technique in plant analysis.

flavonoids; phenolic acids; xanthines; Ilex paraguariensis; TLC

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