engleski

VALIDATION OF HPLC ASSAY FOR THE DETERMINATION OF OXYPRENYLATED ANTHRAQUINONES PRODUCED BY RHAMNUS L. SPECIES

Rhamnus L. spp. (Rhamnaceae) is known to contain active anthraquinones secondary metabolites but the presence of oxyprenylated ones is still not reported. As continuation of our studies [1, 2], a new method [3] was validated to quantify these analytes in plant extracts after extraction with n-hexane and methanol using a ODS column, water and methanol (1% formic acid, v/v) as mobile phase at 0.7 mL min-1 in gradient elution. Quantitative analyses, at 435 nm, revealed LOQ of 0.5 M and linearity up to 125 M. Precision values ranged from 0.2% to 12.9% while trueness from 12.2% to 12.7%.

Rhamnus; anthraquinones; HPLC

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