Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi !

Bio-assays for drug safety assessment: Design, precautions and integration (CROSBI ID 50567)

Prilog u knjizi | izvorni znanstveni rad

Irwin, William A. ; Antolović, Roberto ; Jelić, Dubravko Bio-assays for drug safety assessment: Design, precautions and integration // Medicinal Chemistry in Drug Discovery. Design, Synthesis and Screening / Jelić, Dubravko (ur.). Lahti: Transworld Research Network, 2013. str. 301-326

Podaci o odgovornosti

Irwin, William A. ; Antolović, Roberto ; Jelić, Dubravko

engleski

Bio-assays for drug safety assessment: Design, precautions and integration

High-throughput screening (HTS) is routinely employed in drug discovery to assay pipeline candidates for on-target and off-target responses (i.e. desired biological effects and safety concerns). HTS is often based on measuring thousands of drug candidates per day with a single endpoint assay, often with a single dose of compound, by utilizing absorbance, fluorescence, or luminescence methods. Conversely, microscopy-based methodologies are founded on measuring properties of a limited number of drugs per day, by using several different microscope assays with multiple drug doses, and even with kinetic measurements. FACS (fluorescence activated cell sorting) is another form of multi-channel screening technology. With automatic analysis algorithms/technologies, tedious and time consuming single-point assays can now be automated with both FACS and microscopy-based methods (i.e. mitochondrial membrane potential, lysosomal mass, cell cycle, etc.). This review will discuss the challenging aspects of in vitro bio-assay design, implementation and integration common to plate reader, FACS, microscopy, together with other aspects of screening technologies (i.e. plate layout, drug dosing, dye artifacts, data interpretation, etc.). Mitochondrial assays have become of interest to discovery toxicology, and will have particular emphasis.

HTS, HCS, bioassays, drug safety, drug design

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

301-326.

objavljeno

Podaci o knjizi

Medicinal Chemistry in Drug Discovery. Design, Synthesis and Screening

Jelić, Dubravko

Lahti: Transworld Research Network

2013.

978-81-7895-560-5

Povezanost rada

Temeljne medicinske znanosti, Farmacija, Biologija