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Development and characterization of new polymorphic microsatellite markers for Degenia velebitica (Degen) Hayek(Brassicaceae)

Twelve novel polymorphic microsatellite markers were developed for Degenia velebitica. All 12 microsatellite markers showed single-locus amplification and reveal 88 alleles in 20 individuals from a natural population. The number of alleles per microsatellite locus varied from 3 to 19. The observed heterozygosity, the expected heterozygosity, and polymorphic information content ranged from 0.250 to 0.950, 0.234 to 0.938 and 0, 220 to 0, 908, respectively. The significant deviations from the Hardy–Weinberg expectations were detected in two microsatellite loci. These new microsatellite markers will be a useful tool for studying genetic diversity, genetic structure and phylogeography of D. velebitica, as well as for successful conservation and management of this endangered species.

Brassicaceae  Monotypic genus  Degenia velebitica; Microsatellites;  Endemic; Endangered

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