engleski

Biomimetic approach in bone tissue engineering

Scaffold materials for use in bone tissue engineering that mimic both the structure and mechanical properties of the natural bone still represent a great challenge for researchers. The present work reports on the preparation of poly(ε- caprolactone)-coated highly porous hydroxyapatite scaffolds derived from cuttlefish bone using the vacuum impregnation technique. The results showed that the poly(ε-caprolactone)-coated HAp scaffold resulted in a material with improved mechanical properties that keeps the original interconnected porous structure indispensable for tissue growth and vascularization. The mechanical properties of the composite scaffold are within the lower range of properties reported for human trabecular bones. The preliminary in vitro biological evaluation of the scaffolds carried out using pre-osteoblastic MC3T3-E1 cell line showed that cells adhered well, proliferated and were able to differentiate on the composite scaffold. The microstructure of the composite scaffold allows cell migration through the entire depth of the scaffold after 21 days of culture. These results suggest that poly(ε- caprolactone)-coated hydroxyapatite scaffold derived from cuttlefish bone could be a suitable candidate for bone tissue engineering applications.

cuttlefish bone; poly(ε-caprolactone); hydroxyapatite; scaffold; tissue engineering

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