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TRANSFERABILITY OF REFERENCE INTERVALS FOR SERUM PROTEIN ELECTROPHORESIS FROM CELLULOSE ACETATE TO AGAROSE FOR THE URBAN POPULATION OF ZAGREB, CROATIA

BACKGROUND: Serum protein electrophoresis (SPEP) is an easy assay used for screening of qualitative and quantitative protein pattern abnormalities. Most widely used, depending on media, are SPEP on cellulose acetate, SPEP on agarose and capillary electrophoresis. The aim of our study was to evaluate the transferability of reference intervals (RI) for SPEP determined on cellulose acetate to agarose for the urban population of Zagreb, Croatia. METHODS: The RI for SPEP on cellulose acetate were determined on the 2246 randomly selected urban adults from population of Zagreb, Croatia in the period from 1998 to 2000 using automated system Olympus Fractoscan Junior. The transferability studies were performed for the separation of serum proteins on agarose using semiautomated system Sebia Hydrasys 2 Scan on the reference sample group of 120 healthy adults with standard laboratory tests within RI determined for the Croatian population. RESULTS: RI for adult population for SPEP on cellulose acetate in accordance to agarose were for albumin 57, 0-69, 0% vs. 59, 2–70, 5% ; alpha-1-globulins 2, 0–4, 0% vs. 1, 9–3, 2% ; alpha-2-globulins 5, 0–9-5% vs. 7, 9– 12, 6% ; beta-globulins 7, 5–12, 3% vs. 7, 5–12, 0% and gamma-globulins 12, 7– 22, 1% vs. 9– 16, 3%, respectively. CONCLUSIONS: Our results showed that RI obtained for SPEP on cellulose acetate in accordance to agarose are slightly higher for albumin using agarose, do not differ for alpha-1-globulins and beta-globulins, but there is a great difference for alpha-2-globulins and gamma-globulins.The reason for this could be in fact that in an electric field each protein migrates with a speed which is the result not only of his net charge at given pH buffer, size and shape, but also evaporation current, electroendosmosis and porosity of the media, because of which the RI for SPEP have to be produced for each media separately. The difference in the results of SPEP depending on the media is also apparent in the RI for capillary electrophoresis, which, according to literature, are more like those on cellulose acetate than those on agarose. Therefore, it is important that report of SPEP is standardized, containing data of media used and corresponding RI. It is expected that this would reduce variation between laboratories and the risk of misinterpretation of results.

TRANSFERABILITY OF REFERENCE INTERVALS; SERUM PROTEIN ELECTROPHORESIS; AGAROSE

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