engleski

Gallic, caffeic and tannic acids inhibit tumor growth and angiogenesis in Ehrlich ascites tumor

Reactive oxygen species (ROS) are associated with multiple cellular functions such as cell proliferation, differentiation, apoptosis and angiogenesis. Therefore, inhibition of the ROS, the initial step of tumor growth and angiogenesis, is a promising strategy for cancer chemoprevention and therapy. Gallic (GA), caffeic (CA) and tannic (TA) acids are widely consumed dietary supplements that have antitumor activity. In the present study, we investigated antioxidant, antitumor and anti- angiogenic activities of phenolic acids in Ehrlich ascites tumor (EAT)-bearing mice. EAT cells (2.5 x 106) were implanted intraperitoneally (i.p.) in Swiss albino mice. One day after tumor inoculation, mice were injected i.p. with GA and CA at dose of 40 and 80 mg kg-1 bw or TA at dose of 10 mg kg-1 bw during 10 days. On day 11, ascites volume, the total number of cells, differential count of the cells present in the peritoneal cavity, functional activity of macrophages, anti- angiogenic and antioxidant parameters were determined. The growth of EAT cells and formation of ascites in the peritoneum of EAT- bearing mice was inhibited by test components. Further, results on decrease in the peritoneal angiogenesis and microvessel density show the anti-angiogenic potential of test components in vivo. Taken together, we conclude that phenolic acid may increase the cytotoxic actions of macrophages and inhibit tumor growth and angiogenesis ; inhibitory activity on angiogenesis may be mediated through their anti- oxidative activity.

Gallic acid ; caffeic acid ; tannic acid ; tumor growth ; angiogenesis ; anti-oxidative activity

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