Sex-dependent expression of water channel AQP1 along the rat nephron (CROSBI ID 216660)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Herak-Kramberger, Carol Mirna ; Breljak, Davorka ; Ljubojević, Marija ; Matokanović, Mirela ; Lovrić, Mila ; Rogić, Dunja ; Brzica, Hrvoje ; Vrhovac, Ivana ; Karaica, Dean ; Micek, Vedran ; Ivković Dupor, Jana ; Brown, Dennis ; Sabolić, Ivan
engleski
Sex-dependent expression of water channel AQP1 along the rat nephron
In the mammalian kidney, non-glycosylated and glycosylated forms of water channel protein AQP1 coexist in the luminal and basolateral plasma membranes of proximal tubule and descending thin limb. Factors that influence AQP1 expression in (patho)physiological conditions are poorly known. Thus far only angiotensin II and hypertonicity were found to upregulate AQP1 expression in rat proximal tubule in vivo and in vitro (Am J Physiol Renal Physiol 297:F1575, 2009), a phenomenon that may be relevant for higher blood pressure observed in men and male experimental animals. Here we investigated the sex-dependent AQP1 protein and mRNA expression in the rat kidney by immunochemical methods and qRT-PCR in tissue samples from prepubertal and intact, gonadectomized, and sex hormone-treated gonadectomized adult male and female animals. In adult rats, the overall renal AQP1 protein and mRNA expression was ~80% and ~40% higher, respectively, in males than in females, downregulated by gonadectomy in both sexes, and upregulated strongly by testosterone- and moderately by progesterone-treatment ; estradiol- treatment had no effect. In prepubertal rats, the AQP1 protein expression was low compared to adults and slightly higher in females, whereas the AQP1 mRNA expression was low and similar in both sexes. The observed differences in AQP1 protein expression in various experiments mainly reflect changes in the glycosylated form. The male- dominant expression of renal AQP1 in rats, which develops after puberty largely in the glycosylated form of the protein, may contribute to enhanced fluid reabsorption following the androgen- or progesterone-stimulated activities of sodium- reabsorptive mechanisms in proximal tubules.
Western blotting; aquaporin 1; immunocytochemistry; real time RT-PCR; sex differences
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Podaci o izdanju
308 (8)
2015.
F809-F821
objavljeno
1931-857X
10.1152/ajprenal.00368.2014
Povezanost rada
Temeljne medicinske znanosti, Farmacija