The role of sevoflurane and ionising radiation on the level of primary DNA damage measured in blood and different organs of Swiss albino mice by the alkaline comet assay (CROSBI ID 623277)
Prilog sa skupa u zborniku | izvorni znanstveni rad | domaća recenzija
Podaci o odgovornosti
Benković, Vesna ; Milić, Mirta ; Horvat Knežević, Anica ; Halovanović, Sanja ; Borojević, Nikola ; Oršolić, Nada
engleski
The role of sevoflurane and ionising radiation on the level of primary DNA damage measured in blood and different organs of Swiss albino mice by the alkaline comet assay
Sevoflurane is general anaesthetic suitable for short surgical procedures due to its quick induction of anaesthesia, maintaining spontaneous breathing frequency and hemodynamic stability of patients. However, it can directly trigger the formation of peroxynitrite, significantly increase intracellular levels of H2O2, peroxide, superoxide anion and nitric oxide in peripheral polymorphonuclear neutrophils 1h after the treatment ; lowering the levels of intracellular glutathione, and increase radiosensitivity of cells also exposed to ionising radiation (IR). We wanted to evaluate the level of sinergistic effect and possible radiosensitivity DNA damage in blood, and different organs of Swiss albino mice after exposure to both sevoflurane (2.4 %, 50:50) and the 1Gy gamma-ray radiation generally used in diagnostic purposes after 0, 2, 6 and 24 hours from the combined treatment with alkaline comet assay. Combined exposure to sevoflurane and IR has demonstrated synergistic effect. Due to metabolising of the sevoflurane, there was different sensitivity between blood, liver, kidney and brain cells.
sevoflurane ; ionising radiation ; Swiss albino mice ; comet assay
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Podaci o prilogu
126-131.
2015.
objavljeno
Podaci o matičnoj publikaciji
Proceedings of the tenth Symposium of the Croatian Radiation Protection Association
Petrinec, Branko ; Bituh, Tomislav ; Milić, Mirta ; Kopjar, Nevenka
Zagreb: Denona
1849-5060
Podaci o skupu
10th Symposium of the Croatian Radiation Protection Association with international participation
poster
15.04.2015-17.04.2015
Šibenik, Hrvatska