engleski

Optimization of Supercritical CO2 Extraction of Helichrysum italicum Dried Flowers by Response Surface Methodology: GC-MS Profiles of the Extracts and Essential Oil

Supercritical (SC) CO2 extraction of air-dried Immortelle flowers was performed at different process parameters. The optimal extraction conditions related to the yield were determined by response surface methodology (yield 4.09% at 20 MPa and 52°C). The extracts were analysed by GC-MS and tremetone derivatives dominated: bitalin A, 12-acetoxytremetone, gnaphaliol, 1- [2-(2-methyl-2, 3-dihydroxypropyl)-2, 3- dihydro-1-benzofuran-5-yl]ethanone, isobutyl bitalin A and 1-[2-(acetylprop-1-en-2-yl)-3- hydroxy-2, 3-dihydro-1-benzofuran-5- yl]ethanone. Striking differences were found among the essential oil and SC-CO2 extracts composition. The major oil compounds were γ- curcumene, α-pinene, β-selinene, α-selinene and limonene. Mono- and sesquiterpene were found among minor constituents of the extracts. Neryl acetate was present in the extracts and essential oil.

Helichrysum italicum ; supercritical CO2 extraction ; response surface methodology ; GC-MS profiles of the extracts and essential oil

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