engleski

Impact of weight reduction program on the level of lipid peroxidation

Obesity is strictly linked to changes in redox state ; oxidative stress mainly results from positive energy balance that leads to excessive fat accumulation in non-adipose tissues, with subsequent development of obesity co-morbidities (Savini et al., Int J Mol Sci 2013). The aim of this study was to explore the impact of the weight reduction program on the level of malondialdehide (MDA) well established marker of lipid peroxidation and oxidative stress. Patients at the outpatient Clinic of the Department of Endocrinology, Diabetes and Metabolism disorders at the Dubrava University Hospital were enrolled in the weight reduction program involving diet and exercise. Participants received counseling and education during the initial week and were invited for the follow-up visits after one, three, six and twelve months. The plasma samples were collected at the initial visit and in one year period and were stored at -80 ºC. In plasma samples MDA level was assessed by the use of HPLC. Thirty five obese patients enrolled in the weight reduction program participated in this preliminary study (age 47.9 ± 12.4 (range 21 - 69) ; body weight 109.2 ± 15.3 kg (range 88.5 – 150 kg) ; 77.1 % were woman). At the beginning of the study MDA level was 4.08 ± 1.7 μM (mean ± SD). In one year follow-up, MDA level in the same group of patients was 2.71 ± 0.9 μM, which was statistically lower from the level at the beginning of the study (p < 0.05). Significant decrease in a year period was also observed for the body weight (p < 0.001) with the mean difference between initial and final measurement being 5.5 ± 6.5 kg. The results indicate that weight reduction program involving diet and exercise can reduce level of oxidative stress and consequently lead to healthier life.

obesity; oxidative stress; biomarker; weight reduction program

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