engleski

SOLID PHASE EXTRACTION – SOLUTIONS FOR IMPROVED BIOANALYSIS

Modern bioanalytical laboratories are tasked with providing high-quality analytical results from complex biological samples in a high-throughput environment. The choice of sample preparation technique is important since this is the most time-consuming and labor-intensive process in bioanalysis. The importance of this step in bioanalysis steams from three major concerns – removing interferences from various biological samples matrices, concentrating the analytes of interest, and improving analytical method performance. Conventional sample preparation techniques such as dilution, filtration, liquid-liquid extraction and protein precipitation are now been consider as methods of the past. In bioanalysis, solid phase extraction is usually chosen as one of the best options available for sample preparation as it can provide substantial benefits including low matrix factor, high sample cleanliness, and concentration of low level analytes. Although solid-phase extraction is not novel sample preparation technique, during last decade we have witnessed its rapid development in bioanalysis. Improvements in solid-phase technique have been focused on development of innovative selective sorbens such as moleculary imprinted polymers, immunosorbent, nanoparticles and multi-mode sorbents. Furthermore, formats such as dispersive solid-phase extraction, disposable pipette extraction, 96-well plates, hybrid and twodimensional solid-phase extraction offer a variety of extraction processes and provide unique advantages of bioanalytical methods. On-line solid phase extraction utilizing column switching techniques is rapidly gaining acceptance in bioanalytical applications. The purpose of the present work was to illustrate the importance of sample preparation exemplified by solid-phase extraction for the bioanalytical method development of drugs (immunosuppressives and chemotherapeutic agents) and vitamins.

bioanalysis, SPE, sample preparation

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