engleski

Characterization of olive pomace extract obtained by cyclodextrin-enhanced pulsed ultrasound assisted extraction

Cyclodextrin enhanced pulsed ultrasound assisted extraction (UAE) procedure was developed for obtaining olive pomace (OP) extract rich in bioactive polyphenols, principally oleuropein, tyrosol, and hydroxytyrosol. 20-minute extraction using 12 mm probe at 100% output intensity was selected as optimal for the extraction of OP polyphenols using 60% EtOH at 1:40 solvent to solid ratio. Addition of cyclodextrins (CDs) to extraction solvent (especially randomly-methylated beta CD and hydroxypropyl-beta-CD) improved extraction yields and stability and the appearance (colour and particle size distribution) of obtained extracts was also significantly affected. Extracts obtained under optimized conditions contained 886.46 mgkg-1 of hydroxytyrosol, 1117.02 mgkg-1 of tyrosol and 1744.37 mgkg-1 of oleuropein. In comparison to conventional solvent extraction, application of UAE resulted in significantly higher yields reduced extraction time and solvent consumption.

olive pomace ; ultrasound assisted extraction ; cyclodextrin ; tyrosol ; hydroxytyrosol ; oleuropein

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