engleski

Phytophthora species associated with woody plants in Macedonia

Surveys were carried out in 15 sweet chestnut populations, 15 apple orchards and 7 forest and ornamental nurseries throughout Macedonia for presence of collar and root lesions, rot and crow n symptoms on trees and seedlings. Over 350 soil, and 50 bark samples were collected from both symptomatic and asymptomatic plants. Samples were baited using leaves of Prunus laurocerasus, Fagus moesiaca, Quercus spp. and Malus spp., and used V8 PARPNH and CMA+ selective media for isolations. DNA was extracted from cultures of 255 isolates, amplified by PCR using ITS4 and ITS6 universal Phytophthora primers and the products sequenced. Comparisons with sequences in a Phytophthora database identified 7 isolates of P. cambivora, all from chestnut trees or related soil samples, 1 of P. cinnamomi (Chamaecyparis lawsoniana - nursery) and 1 isolate of P. gonapodyides (beech). Five isolates of P. cactorum, 3 of P. megasperma, 2 of P. plurivora, 1 of P. rosacearum and 1 of P. citricola were obtained from apple trees or orchard soils. This report is the first to identify these species in Macedonia.

Collar rot, root rot, lesions, DNA, Phytophthora

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