Phytopthora spp. isolated from chestnut populations in the Republic of Macedonia (CROSBI ID 652924)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Risteski, Mihajlo ; Kuzmanovska, Biljana ; Rigling, Daniel ; Ježić, Marin ; Sotirovski, Kiril
engleski
Phytopthora spp. isolated from chestnut populations in the Republic of Macedonia
Symptoms specific for Phytophthora spp. on sweet chestnut (Castanea sativa) have been previously reported in Macedonia, but have never been confirmed by isolation of cultures or molecular techniques. We surveyed 15 chestnut populations, aged 15 to 70 years, at sites: Skudrinje, Osoj, Knezino, Straza, Recane. Vrutok, Kale, Kalista, Trebenista, Vratnica and Smolari throughout Macedonia. We assessed for presence of collar and root rot and lesions, as well as crown and seedlings symptoms. Over 130 soil and bark samples were collected from both symptomatic and asymptomatic chestnut plants. Using the bait method, and culturing on PARPNH and CMA+ Phytophthora-selective nutritious media, we isolated 71 Phytophthora-like cultures. We extracted DNA from all cultures, and DNA samples were amplified and sequenced using ITS4 and ITS 6 universal Phytophthora primers. Using online Phytophthora database blast, we identified 5 isolates of P. cambivora and 4 of P. cactorurn. We tested the pathogenicity of one random isolate representative for each of the two species, on excised chestnut sticks collected from a single coppice. We tested on 40 sticks per isolate, 20 per two sets of diameters (5-10 mm and 10-15 mm) of the same length (10 cm). The tests pointed out P.cactorum as more pathogenic than P. cambivora, both on thicker and thinner sticks. This is the first report in Macedonia about identifying these species through cultures and partial sequencing and assessment of their pathogenicity.
chestnut populations, disease, FLO
nije evidentirano
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Podaci o prilogu
47-47.
2017.
objavljeno
Podaci o matičnoj publikaciji
Book of Abstracts "Sustainable Forestry - Fact or Fiction?"
Skopje: Ss. Cyril and Methodius University
978-9989-132-19-3
Podaci o skupu
Sustainable Forestry - Fact or Fiction?
poster
04.10.2017-06.10.2017
Skopje, Sjeverna Makedonija