hrvatski

Izvanstanični Hsp70 - od mehanizma djelovanja do potencijalnog dijagnostičkog biljega

Heat shock proteins (Hsps) are highly conserved and ubiquitously expressed proteins that play an essential role as molecular chaperones in maintaining the homeostasis of cells and tissues. The members of the Hsp superfamily are categorised into several families on the basis of the approximate molecular weight. The best described Hsp70 family member is stress-inducible 72-kDa protein with cellular functions including preventing protein aggregation, facilitating protein refolding, and chaperoning proteins, all of which improve survival of a cell faced with stressors. During cellular stress, intracellular Hsp70 levels increase to provide cellular protection. The induction of Hsp70 has to be tightly controlled, since its persistent presence would adversely affect protein homeostasis and intracellular functions, leading to inappropriate growth control and possibly cell death. To date, Hsp70 has mainly been studied as intracellular chaperone that is released from cells only after severe damage that causes cell lysis. It is now also known that Hsp70 can be secreted from a variety of cell types, even when these cells are completely viable, acting as a damage-associated molecular pattern (DAMP). People suffering from a variety of inflammatory and infective diseases have chronically elevated basal levels of extracellular Hsp70 (eHsp70) relative to healthy, aged-matched controls. In addition, an increase of eHsp70 in the blood occurs in healthy organisms after exposure to acute stressors. Chronic obstructive pulmonary disease (COPD) is a heterogeneous disease with many comorbid and confounding conditions, and markers of disease may vary from one phenotype to another. Therefore, exploration of systemic inflammatory parameters will provide new information on the systemic inflammatory COPD phenotype that might need more specific and more personalized therapeutic strategy. This might improve relevant clinical outcomes of the disease. The lecture will discuss the mechanism of action of eHsp70 and its potential diagnostic significance, especially in COPD.

izvanstanični Hsp70, kronična opstrukcijska plućna bolest

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