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Short –therm stability of routine haematology parameters after refrigeration.

BACKGROUND-AIM: Storage time and temperature may significantly affect routine haematology results. There is no unambiguous criteria for whole blood stability at 2-8°C. Our aim was to determine short-therm stability of routine haematology parameters in whole blood samples stored in different time intervals within 48 hours at 2-8 °C. METHODS: Blood was collected from 20 patients using K3EDTA Vacuette tubes (Greiner Bio-One GmbH, Kremsmünster, Austria). Routine haematology parameters were measured using the Sysmex XT 1800i analyzer (Sysmex Corporation, Kobe, Japan) immediately upon receipt. Whole blood samples where stored at 2-8°C and re- analysed after a 6, 12, 24 and 48 hours after baseline measurements. Paired samples t-test was used for statistical comparisons to baseline results. The level of significance was set at P<0.05. Mean biases from baseline results were compared to Ricos desirable biological specifications to define clinically relevant variations. RESULTS: Statistically different results compared to baseline values were found for: WBC after 6, 12 and 48 hours (P=0.008, P=0.035 and P=0.012, respectively) ; hematocrit after 48 hours (P=0.031) ; MCV after 24 and 48 hours (P=0.008 and P<0.001) ; MCHC after 48 hours (P<0.001) ; platelets after 6 hours (P=0.038) ; MPV after 6, 12, 24 and 48 hours (P<0.001) ; neutrophils (%), lymphocytes (%) and eosinophils (%) after 48 hours storage (P=0.001, P=0.016 and P=0.015, respectively). MPV exceeded the predefined criteria of 2.3% for clinical significance in all the time points tested (4.9% after 6 ; 7.5% after 12 ; 10.3% after 24 and 14.1% after 48 hours). WBC, MCHC and lymphocytes (%) exceeded the criteria for desirable bias after 24 hours storage. CONCLUSION: All the routine haematology parameters tested, except MPV, showed acceptable stability when stored at 2-8 °C within 24 hours, compared to predefined desirable specification for bias in our laboratory conditions.

temperature stability, hematologic parameters, hematologic analyser

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