Mathematical model of the MenD-catalyzed 1,4-addition (Stetter Reaction) of α-ketoglutaric acid to acrylonitrile (CROSBI ID 247204)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Sudar, Martina ; Vasić-Rački, Đurđa ; Müller, Michael ; Walter, Alexandra ; Findrik Blažević, Zvjezdana
engleski
Mathematical model of the MenD-catalyzed 1,4-addition (Stetter Reaction) of α-ketoglutaric acid to acrylonitrile
The Stetter reaction, a conjugate umpolung reaction, is well known for cyanide-catalyzed transformations of mostly aromatic aldehydes. Enzymatic Stetter reactions, however, have been largely unexplored, especially with respect to preparative transformations. We have investigated the kinetics of the MenD-catalyzed 1, 4-addition of -ketoglutaric acid to acrylonitrile which has shown that acrylonitrile, while an interesting candidate, is a poor substrate for MenD due to low affinity of the enzyme for this substrate. The kinetic model of the reaction was simplified to double substrate Michaelis–Menten kinetics where the reaction rate linearly depends on acrylonitrile concentration. Experiments at different initial concentrations of acrylonitrile under batch, repetitive batch, and fed-batch reactor conditions were carried out to validate the developed mathematical model. Thiamine diphosphate dependent MenD proved to be quite a robust enzyme ; nevertheless, enzyme operational stability decay occurs in the reactor. The spontaneous reactivity of acrylonitrile towards polymerization was also taken into account during mathematical modeling. Almost quantitative conversion of acrylonitrile was achieved in all batch reactor experiments, while the yield of the desired product was dependent on initial acrylonitrile concentration (i.e., the concentration of the stabilizer additive). Using the optimized reactor parameters, it was possible to synthesize the product, 6-cyano-4-oxohexanoic acid, in a concentration of 250 mM. The highest concentration of product was achieved in a repetitive batch reactor experiment. A fed- batch reactor experiment also delivered promising results, especially regarding the short reaction time needed to achieve a 200 mM concentration of product. Hence, the enzymatic Stetter reaction with a highly reactive acceptor substrate can be performed on a preparative scale, which should enable similar transformations with acrylate, methacrylate, and methyl vinyl ketone.
enzyme ; carboligation ; kinetic model ; optimization ; reactor selection
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Podaci o izdanju
268
2018.
71-80
objavljeno
0168-1656
1873-4863
10.1016/j.jbiotec.2018.01.013
Trošak objave rada u otvorenom pristupu
Povezanost rada
Biotehnologija, Kemijsko inženjerstvo