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Dietary intake and antitumour preventive properties of 2, 4, 6-trifluorophenylboronic acid in mice: Some results and prospects (CROSBI ID 662416)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Marasović, Maja ; Miloš, Mladen ; Stojković, Ranko ; Ivanković, Siniša Dietary intake and antitumour preventive properties of 2, 4, 6-trifluorophenylboronic acid in mice: Some results and prospects // 6th Food Safety Congress : Abstract book. Istanbul: Food Safety Congress, 2018

Podaci o odgovornosti

Marasović, Maja ; Miloš, Mladen ; Stojković, Ranko ; Ivanković, Siniša

engleski

Dietary intake and antitumour preventive properties of 2, 4, 6-trifluorophenylboronic acid in mice: Some results and prospects

Research hypothesis Diverse foods as spices, nuts, fruit, vegetables, wine and beer are particularly rich in boron compounds. The mechanisms of their biologic activities are uncertain and have not been sufficiently researched. As far as possible pharmaceutical applications are concerned there are reports of claims that boron compounds alleviates some enzyme inhibition and anticancer properties. Methods Two mouse strains, BALB/c syngeneic with 4T1 cells and C3H/H syngeneic with SCCVII cells, were used. The animals were 3-months-old and weighed 20 – 23 g. They were provided standard diet (Mucedola S.R.L., Milan, Italy) and tap water ad libitum. The animals were kept under conventional conditions: 12/12 h light/dark rhythms, 22°C temperature and 55% humidity. Animals were treated according to the Animal Welfare Regulations. Each experimental group consisted of seven animals. Mouse tumour models of mammary adenocarcinoma 4T1 and squamous cell carcinoma SCCVII were established by injecting 5 x 10⁵ tumour cells subcutaneously into the right thighs of syngeneic mice. For evaluation of cell viability and cytotoxic effects of 2, 4, 6-trifluorophenylboronic acid (3FPBA) on cultured cells, a crystal violet staining assays was used. This test only determines living cells at the time of fixation and staining of the examined samples, while the dead cells are removed by washing of cell cultures. Experiments were carried out in 96-well micro-titre plates ; 1 x 10⁴ tumour cells/250 μl medium were added to each well. Results In vitro the cytotoxic activities of 3FPBA at different concentrations 0.1, 1.0 and 10 mg/ml were evaluated on mouse mammary adenocarcinoma 4T1 and squamous cell carcinoma SCCVII cell lines and on hamster lung fibroblast V79 and mouse dermal fibroblasts L929 cell lines. Generally, the cytotoxic effect of 3FPBA was dose-dependent. In all tested tumour and non-tumour cell lines, 10 mg/ml 3FPBA significantly reduced the number of surviving cells compared with the control group. Antitumour activity in vivo was assessed on the same tumour models as the in vitro experiments. Preliminary experiments showed that the intra- peritoneal and intra-tumoural application of 50 mg/kg of 3FPBA in a single dose was well-tolerated by all mice. When 3FPBA was administered per-oral, in five consecutive days in five doses of 50 mg/kg, it was also well-tolerated by all mice, i.e. the animals were generally in good condition and did not show a significant weight loss. 3FPBA do not appear to have intrinsic toxicity issues and the metabolic end product is considered non- toxic to mice. The effects of per-oral and intra- peritoneal (i.p.) applications of 3FPBA on the growth of mammary adenocarcinoma 4T1cells showed significantly slowed tumour growth compared with control, whereas no significant delay in tumour growth was observed when 3FPBA was administered in mice with transplanted squamous cell carcinoma SCCVII cell lines. The different measured tumour parameters, after transplantation of mammary adenocarcinoma 4T1 and squamous carcinoma SCCVII cells in syngeneic mice, without and after treatment by 3FPBA, clearly show that 3FPBA has antitumour activity in adenocarcinoma 4T1 cell lines, especially when administered per-oral. For treated mice versus control, the difference in volumes, after the ninth day of the p.o. treatment was 500 mm³, which represents a 60% tumour growth inhibition and 7 days tumour growth delay. Discussion Our work showed that 3FPBA proved to be highly cytotoxic on mammary adenocarcinoma 4T1cells cells and even after repeated per-oral administration in large doses, was well-tolerated by mice. Determination of cytotoxic activity in vitro showed that growth inhibition LC 50 values were estimated at 4.5 – 7.5 mg/ml. Funding This work has been supported in part by Croatian Science Foundation under the project HRZZ-IP-2014- 09-6897.

Boron compounds ; antitumour ; 4T1 and SCCVII cells ; diet

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Podaci o prilogu

0096

2018.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

6th Food Safety Congress : Abstract book

Istanbul: Food Safety Congress

Podaci o skupu

6th Food Safety Congress

poster

03.05.2018-04.05.2018

Istanbul, Turska

Povezanost rada

Kemija, Nutricionizam, Veterinarska medicina