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NMR study of nano-bio interface: A case of interaction between gold nanoparticles and biothiols (CROSBI ID 663315)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Pem, Barbara ; Vrček, Valerije ; Ćurlin, Marija ; Domazet Jurašin, Darija ; Vinković Vrček, Ivana NMR study of nano-bio interface: A case of interaction between gold nanoparticles and biothiols // Adriatic NMR Conference : Book of abstracts / Bregović, Nikola ; Namjesnik, Danijel ; Novak, Predrag et al. (ur.). Zagreb: Odsjek za kemiju, Prirodoslovno-matematički fakultet, Sveučilište u Zagrebu, 2018. str. 49-49

Podaci o odgovornosti

Pem, Barbara ; Vrček, Valerije ; Ćurlin, Marija ; Domazet Jurašin, Darija ; Vinković Vrček, Ivana

engleski

NMR study of nano-bio interface: A case of interaction between gold nanoparticles and biothiols

The growing interest in utilization of gold nanoparticles (AuNPs) for diagnostics and treatment of various diseases [1] generates the need to examine their interactions with biological systems. Upon contact with biological media, NPs are immediately coated with a layer of adsorbed biomolecules called “corona” [2]. However, detailed knowledge still lacks about the nature and mechanisms behind the nano-bio interactions. One of the possible binding mechanisms is through the thiol groups, considering the high affinity of sulphur for metals. Endogenous biothiols such as cysteine and glutathione are therefore useful as models for studying the mechanism of nano-bio interactions [3]. In this study, 1H NMR was employed to study the interactions of AuNPs with cysteine and glutathione. Nanoparticles were synthesized by reduction of tetrachloroauric acid with sodium borohydride in the presence of cysteine or glutathione as stabilizing agents. 1H NMR spectra were recorded immediately after mixing the reagents, and subsequently every 20 minutes until AuNPs formation finished. Process of interaction between CYS and GSH with the AuNPs surface was observed by recording chemical shifts of signals corresponding to protons close to the thiol group, along with the loss of resolution and peak broadening. Furthermore, the intermediate spectra were captured, with distinct peaks corresponding to both bound and unbound biothiols. The results suggest the thiol group of CYS and GSH is the most responsible for biothiols-nanoparticle binding. In addition, AuNPs stabilized with CYS and GSH were characterized by means of size distribution, surface charge and visualized by transmission electron microscopy.

gold nanoparticles ; biothiols ; NMR ; cysteine ; glutathione

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Podaci o prilogu

49-49.

2018.

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objavljeno

978-953-6076-42-0

Podaci o matičnoj publikaciji

Adriatic NMR Conference : Book of abstracts

Bregović, Nikola ; Namjesnik, Danijel ; Novak, Predrag ; Pičuljan, Katarina

Zagreb: Odsjek za kemiju, Prirodoslovno-matematički fakultet, Sveučilište u Zagrebu

Podaci o skupu

Adriatic NMR 2018

poster

15.06.2018-17.06.2018

Mali Ston, Hrvatska

Povezanost rada

Biologija, Farmacija, Kemija