engleski

Post-flood patterns of indoor samples of fumonisin B2 producing black Aspergilli

Background. Black Aspergilli are ubiquitous moulds which contaminate foodstuffs and occur in indoor environments. Recent studies showed that some species from the section produce several fumonisin isomers including FB2 that could contribute to irritant, allergic and toxic effects. Methodology. Black Aspergilli were isolated from the airsamples (N=60) and dust (N=60) collected during winter and summer of 2016 and 2017 in flood affected area and control area. Identification to the species level was based on the partial calmodulin sequences. FB2 producing abilities were tested by analysing prepared microextracts of an each isolate using LC/MS. Results and conclusion. In the flood affected area the highest concentration of airborne black Aspergilli was observed in winter period and their concentrations were 50 times higher than in control area (987 ± 1154 CFU/m3). Their concentration in dust was similar in flood affected and control area. 49/85 analysed black Aspergilli was from the samples collected in flood affected area. FB2 was detected in 4 extracted airborne and 11 dustborne black Aspergilli in concentrations 0.617 ± 0.582 µg/ml and 9.237 ± 15.092 µg/ml and thus may contribute to the harmful effects in conditions of increased concentration of black Aspergilli, especially in flood affected area.

Black Aspergilli, fumonisin B2, indoor fungi, post-flood

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